results the results showed that all the strains had the dominant allele ptxs1a. there were differences between the alleles of the clinical strains and the vaccine strain. conclusions in recent years, a significant increase in the incidence of pertussis has been reported worldwide. our findings regarding the allelic shift of the ptxs1 gene are similar to those reported in many european and ameri...

background and aims: pertussis is a highly contagious, vaccine-preventable disease. determination of the seroepidemiology of pertussis makes possible the evaluation of pertussis immunity in a population. in this study, we determined the seroprevalence of bordetella pertussis igg antibodies in different age groups in tehran, iran. materials and methods: overall, 1101 subjects between ages of 8 ...

background and objective: rapid diagnosis of pertussis is important for the timely isolation of the infection source and early prevention measures among the contact persons, especially among non-vaccinated infants for whom pertussis is life- threatening. materials and methods: targets is481 , is1001, bp0026 and human gapdh gene were used to develop a multiplex real- time pcr assay based on the ...

bordetella pertussis is a gram negative bacterium that causes respiratory tract infection in human (whooping cough). pertussis toxin (pt) is the main component of current acellular pertussis vaccine and the s1 (subunit1) is the main immunogenic part of it. thus, s1 has been the target of many studies as a potent candidate of acellular vaccine against bordetella pertussis, lacking the side effec...

Pertussis is routinely diagnosed with real-time PCR based on insertion sequence IS481, which is not specific for Bordetella pertussis. We conducted a retrospective study using real-time PCRs specific for Bordetella pertussis and for Bordetella holmesii on 177 samples positive for IS481 PCR. Bordetella holmesii DNA was detected in 20.3% samples collected from adolescents and adults.

Bordetella pertussis is the causative agent of pertussis. Here, we report the genome sequence of Bordetella pertussis strain CS, isolated from an infant patient in Beijing and widely used as a vaccine strain for production of an acellular pertussis vaccine in China.

Bordetella pertussis 18323 produces a bvg-regulated 39.1-kDa porin-like protein, OmpQ. OmpQ had 61% similarity to the major porin of B. pertussis and contains conserved regions common to both the neisserial and enteric porin families. The results of Southern blot analysis indicate that strains of Bordetella parapertussis and Bordetella bronchiseptica but not Bordetella avium contain this gene.

We report the prevalence in Bordetella bronchiseptica of IS481, a frequent target for diagnosis of Bordetella pertussis, as approximately 5%. However, PCR amplicons of the predicted size were detectable in 78% of IS481-negative strains. Our results suggest that PCR targeting IS481 may not be sufficiently specific for reliable identification of B. pertussis.

results among the specimens, 11 cases (1.4%) were culture-positive. among these isolates, only two isolates had high mic values for erythromycin and clarithromycin. pulsed-field gel electrophoresis analysis of the isolates revealed 6 pfge profiles (a-f) among which three and two isolates had the same patterns in profiles a and b, respectively. conclusions azithromycin can be a good drug of choi...